CAMK2A Antikörper (pThr306)

Produktnummer ABIN6254013

Der Kaninchen Polyklonal Anti-CAMK2A Antikörper wurde für WB und ELISA validiert. Er ist geeignet, CAMK2A in Proben von Ratte zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für CAMK2A Antikörper (pThr306) (ABIN6254013)

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CAMK2A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-CAMK2A Antikörper

Bindungsspezifität: pThr306

Spezifität: Specific for endogenous levels of the ~50 kDa α-CaM Kinase II and the ~60 kDa β-CaM Kinase II proteins phosphorylated at Thr306. Immunolabeling is completely eliminated by treatment with λ-phosphatase. It has been reported that this antibody may recognize some level of non-phosphorylated pure recombinant protein, but in our hands (as shown in our WB image) in native tissue, the antibody reacts in a phospho-specific manner.

Kreuzreaktivität: Maus, Ratte

Homologie: bovine, canine, chicken, human, Xenopus, Zebrafish

Produktmerkmale: Our CaM Kinase II (Thr306) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects CaM Kinase II (Thr306) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Aufreinigung: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr306 of rat CaMKII, conjugated to keyhole limpet hemocyanin (KLH).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Dilution Range: WB Brain: 1:1000

Dilution Range: WB: 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Lagerung: -20 °C

Informationen zur Lagerung: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Haltbarkeit: 12 months

Referenzen für anti-CAMK2A Antikörper (ABIN6254013)

Bhattacharyya, Lee, Muratcioglu, Qiu, Nyayapati, Schulman, Groves, Kuriyan: "Flexible linkers in CaMKII control the balance between activating and inhibitory autophosphorylation." in: eLife, Vol. 9, (2020) (PubMed).

Details zu CAMK2A

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Andere Bezeichnung: CaM Kinase II

Hintergrund:

Synonyms: Calcium/calmodulin dependent protein kinase II alpha antibody, Calcium/calmodulin dependent protein kinase II beta antibody, Calcium/calmodulin dependent protein kinase II delta antibody, Calcium/calmodulin dependent protein kinase II gamma antibody, Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody, CaM kinase II alpha antibody, CaM kinase II antibody, CaM kinase II beta antibody, CaM kinase II delta antibody, CaM kinase II gamma antibody, CaM kinase II subunit alpha antibody, CaMK-II subunit alpha antibody, CAMK2 antibody, Camk2a antibody, CAMK2B antibody, CAMK2D antibody, CAMK2G antibody, CAMKA antibody, KCC2A_HUMAN antibody

Description Logic: Our CaM Kinase II (Thr306) rabbit polyclonal phosphospecific primary antibody is produced . It detects CaM Kinase II (Thr306) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Gene Name Alternatives: CAMK2A/B

Molekulargewicht: 50 kDa/60 kDa

Gen-ID: 25400

UniProt: P11310

Pathways: WNT Signalweg, Interferon-gamma Pathway, Myometrial Relaxation and Contraction